spectramax 340. Title: SPECTRAmax 340 Microplate Reader manual Author: Research Facilities Keywords: spectramax, 340, microplate reader, manual, Created Date: 12/13/2005 9:07:53 AMSpectraMax Multi-Mode Microplate Readers. spectramax 340

 
Title: SPECTRAmax 340 Microplate Reader manual Author: Research Facilities Keywords: spectramax, 340, microplate reader, manual, Created Date: 12/13/2005 9:07:53 AMSpectraMax Multi-Mode Microplate Readersspectramax 340  AoSMCs were seeded into 6-well culture plates

Call 1-800-745-2710. ELISA plates were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Spectramax 340pc User Manual, Hudson River Saranac Pellet Stove Manual, Alesis Midiverb 1 Manual, User Manual For Samsung Galaxy Ace Duos S6802, Di Style Manual, R150f Transmission Repair Manual, Eos5d Mark 2 Manual. Column chromatography was performed on silica gel (Kieselgel 60, 70–230 mesh, Merck). Percent Transmission Absorbance Competitor 0. Pharmacokinetic and pharmacodynamic analysis. Driessen*,MaaikeA. using SpectraMax i3 (Molecular devices, San Jose, USA). 340-850 nm: Wavelength Selection: Monochromator, turnable in 1 nm increments:. 4. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. Rate of fibrinolysis. Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. SpectraMax 340 from Molecular Devices). When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The Spectra Catalina 340z produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize yacht with two to four people onboard. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Screening for Antigen-Specific IgG Producing Clones by Capture ELISA. Revvity. PerkinElmer. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Summary of Contents for Molecular Devices SPECTRAmax 340PC. VersaMax reads 96-well and strip-well plates. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Data were expressed as mean percentage of viable cells. The VersaMax and SpectraMax 190 read 96-well microplates. Molecular Devices SpectraMax Gemini XS Microplate Reader, 250-850nm, Molecular Devices, for detection of samples in microplates. SpectraMax 190 reads 96-well and strip-well plates. Also for: Spectramax 190, Spectramax 340pc384, Versamax. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Fibrin clots were formed as described above with some modifications 46. Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. The ODs of wells loaded with DMEM plus 10% CS were subtracted as background. 1% N-1-naphtylethylenediamine dihydrochloride, 2. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). Features showcased by this model include. 5% (w/v) sodium dodecyl sulfate-polyacrylamide gels, and transferred to immobilon polyvinylidenedifluoride membranes (Millipore, Bedford, USA). This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. 0200-0729. PGD2 concentrations in samples from treated cell cultures were determined using the linear range of a standard curve and are presented as percentages of the PGD2 levels measured in the solvent controls. The values were corrected for nonspecific background by subtracting the OD 650. The absorbances were transformed to velocity of substrate consumption (nmol/min). Unit powers. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. The cell concentration was measured by a spectrometer (SpectraMax 340, Molecular Devices, USA) with an optical density at 600 nm (OD 600). PC12 or BV-2 cells treated with various concentrations of BBD were stressed with hypoxia for one hour and the supernatant was then assayed for LDH activity. Samples were considered “positive” if the absorbance value was greater than two times the average absorbance value of the negative control wells . This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. 1x10 5 transduced, GFP + SK-NEP-1. 1 mL of culture media with 10% FBS. When necessary, samples were. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Briefly, cells were sequentially treated with terminal deoxynucleotidyl. Supplier: Molecular Devices. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode Opening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. For cell permeabilization, 1 ml of Z buffer (60 mM Na 2 HPO 4 z 7H 2 O, 40 mM NaH 2 PO 4 z H 2 O, 10 mM KCl. \r plates can be used in the SPECTRAmax PLUS 384. Samples containing 25 μg of protein were separated on 12. Donor: 620 nm. This was followed by an additional incubation at 37 °C for 30–60 min. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. 6) and 0. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). These lab workhorses are used in drug discovery, research, bioassay validation, and. #N207) LAL Reagent Water (Lonza cat. Includes: Computer Loaded with Softmax Pro 5. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). 0, MSD) or Spectramax 340 (Molecular Devices). Each experiment was performed in duplicate. • TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027: EX range: 340/80 nm EM range: 616/10 nm: High fg levels of Streptavidin: High. The GH Pro 340 Supplemental Greenhouse LED grow lighting is designed with greenhouse operations in mind. Molecular Device M2e. S. PerkinElmer offers a variety of Microplates For Absorbance and Colorimetric Assays. We also offer depot Repair Service from our base of operations in Southern California (San Diego). Following incubation, 100 μl of solution was transferred to new wells and the color change in the solution was measured with a microtiter plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Continuous kinetic monitoring of enzyme activity was performed on SPECTRAmax 340 or Flexstation 2-384 microplate reader (Molecular Devices, Sunnyvale, CA, USA) and controlled by the Softmax software. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. 30. Calculating % of total mediator release in RBL assay. Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter $1,000. Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . 4- to 5-weeks old female BALB/c mice were purchased from Charles River Laboratories (QC, Canada). 5. Data were expressed as the mean percent viable cell vs. Assay setup for a 384-well low-volume plate. Heme was preincubated for 5 min with A when added before the metals. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 3 1. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. About The SpectraMax GEMINI EM is controlled by an external computer running SoftMax ® Pro software which provides integrated instrument control, data display, and statistical data analysis. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 340 nm / 70 nm. SoftMax Pro. application or budget. This assay measures the two major sugars in wine, glucose and fructose. Bertin Precellys Evolution Touch Homogenizer. Stat Fax 4700 Microstrip Reader. The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. These robust readers have been placed in labs from Antarctica to the. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. Male 8 pin mini Din to Female 9D. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. Immunofluorescence and Aggregation Analysis. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). G. 340/70 nm. 00. It includes both a lamp and a new lamp holder. VersaMax reads 96-well and strip-well plates. 2. 4. Other technologies such as imaging. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. 0. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. 9000-0756. 5mM), and changes in OD 405 nm. mums. Cell permeabilization. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. Operators can conveniently select up to 6 wavelengths at a time for. SpectraMax® M2/M2e user guide a multi-detection microplate reader with two-mode cuvette port SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. Finally, the absorbance at 450 nM was taken using a SpectraMax ® 340 ELISA plate reader (Molecular Devices, USA). Service parts for these models are not being produced and are no longer in stock. 2. Immunofluorescence and Aggregation Analysis. Molecular Devices’ PathCheck® sensor technology. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Following HRP-conjugated secondary antibodies, goat anti-rat IgG (Kirkegaard & Perry. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). The OD 490 was measured with an absorbance microplate reader (SpectraMax 340; Molecular Devices). Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects andSPECTRAmax ® PLUS 384 Microplate Spectrophotometer. Temperature Range: Ambient + 4 °C up to 45 °C. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. #N207) LAL Reagent Water (Lonza cat. 3: The cuvette chamber. Background: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ). The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 20. The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. ISM Equipment Classification (Group 1, Class A) This equipment is designated as. To screen for antigen specific IgG, 50 μL/well of biotinylated antigen at a concentration of 20-50 ng/mL was added and incubated for one hour at RT. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Labsystems Luminoskan RT Microplate Reader. Protein electrophoresis system (BioRad, Hercules, CA) and Odyssey Infrared Imaging System (Li-Cor Biosciences, Lincoln, NE) were used in western blot. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). Homeostatic model assessment for insulin. 5 hours, and is measured in a 96-well plate spectrophotometer at 405 nm (SpectraMax 340; Molecular Devices LLC, Sunnyvale, CA, USA). Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. The maximum specific growth rate μ was calculated from the exponential growth phase ( 52 ) and plotted against the concentration of the different drugs. SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. Italic and boldface type are used for emphasis. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). Nitric Oxide Assay. Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. 2. Example: [Drawer]. TGFα release was calculated as described in the Results. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. The activity of glutathione peroxidase (GPX) was quantified spectrophotometrically at 340 nm, following the method of Takeda et al. Crowder, Ziqian Zeng, Alissa N. Other technologies such as imaging. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Western blot. Data were expressed as the mean LPS-treated cells (100%) vs. Absorbance was read at 450 nm using a Molecular Device Spectramax 340. Data were expressed as the mean percent viable cell vs. Softmax PRO v5. , Sunnyvale CA). Emission. Optical density was determined at 490 nm (background values measured at 690 nm were subtracted) using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. Figure 1. The results are expressed as the percentage inhibition of BrdU incorporation compared to the untreated control. Anti-proteolytic activity. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. TheSpectraMax®Plus384addstheabilitytoread SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. The amount of liberated pNA product during incubations was determined using a standard curve obtained at 410 nm for various concentrations of a pNA stock solution in the SpectraMax 340 microplate reader. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). Plasma glucose levels were measured using FreeStyle Lite (Abbott Diabetes Care Ltd. edu Contact Phone: 216-368-8962. Molecular Devices SpectraMax M5e Microplate Reader. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. 5 g/L-EDTA 0. The OD for each serum is adjusted by subtracting either the OD for spontaneous mediator release or antigen-specific mediator release. TDP-43 stress granular cells were treated with 70% ice-cold. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. The light features a slim profile to prevent. Unit has several areas of damaged plastic. Fig. Analysis of ALA by Ehrlich’s Reagent Assay. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. A. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Created Date: 20051213090753Zmeasured using a SpectraMax 340 multiwell plate. Wavelength Range. Create custom fluorescence assays without having to purchase expensive filter sets. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise results. 2. SPECTRAmax® 190. ZERO BIAS - scores, article reviews, protocol conditions and moreAbsorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. 04. Amino Acid Analysis. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. N-34-LB. 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. 06 ng/mL; FSH: 0. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). Details. Cytotoxicity was determined by measuring the release of LDH. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Related Products: SpectraMax 340 Absorbance Microplate Reader, Molecular Devices SpectraMax 384 Plus UV/Vis Microplate Reader, Molecular DevicesGETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systems450 nm using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). If depot service is the best option for you we will assist you with logistics to help you keep. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. , Sunnyvale, CA). TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. NO release assayThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Briefly, ear or dorsal skin was dissected and fixed with 10% phosphate buffered formalin for 24–72 h. The extreme flexibility and high sensitivity of the SpectraMax GEMINI EM make it appropriate for applications within the fields of bio-A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. Triton X-100 (see Note 2). The absorbances were being measured at 515 nm. Call 1-800-745-2710. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. . The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the. The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. plates can be used in the SPECTRAmax PLUS 384. One plate carrier adapter is provided with the instrument. using SpectraMax i3 (Molecular devices, San Jose, USA). Molecular Devices Spectramax 340PC instructions. Read one sample or up to 384. Microscopy. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. Service Contracts, Preventative Maintenance and Performance. To. SpectraMax Multi-Mode Microplate Readers. The narrow bandwidth used by the model increases measurement accuracy and broad analysis over. Samples were plated in duplicate (r=0. Water – distilled water or equivalent. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. Centrifuge – Eppendorf 5417C with rotor FA 45–30–11. 30. Alves, Amelia K. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 5. The concentration of TNFα in the experimental. , San Jose, CA) using Softmax Pro 7 software. Since the formation of NADPH is stoichiometrically related to the oxidation of glucose-6-phosphate, measurements for glucose + fructose can be determined by the absorbance of NADPH at 340 nm. Donor filter: 616/10 nm. MCE has not independently confirmed the accuracy of these methods. This reader only allows high performance HTRF measurement when assays are run in. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. 3 mm. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. 6. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Filterless readers provide consistent results with steady temperature regulation. edu Contact Phone: 216-368-8962. 2. addresses the needs of life science researchers who quantify very small amounts of DNA, RNA, oligos, and proteins. Products : SpectraMax Plus 384 reader,. All assays were performed in triplicate, and the absorbance at 405 nm was measured using a SpectraMax 340 multiwell plate reader (Molecular Devices). 314 low lamp level warning. SpectraMax Multi-Mode Microplate Readers. 3. 0. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. ) at 490 nm. 3 0. Absorbance values were measured using a plate reader (Molecular Devices; SpectraMax 340) at λ = 405 nm. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. The mixture changed to a red color by a reaction of LDH that was measured by a plate reader (SpectraMax 340 PC; Molecular Devices, San Jose, CA, USA) at a visible wavelength of. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . Molecular Devices SpectraMax 340 PC 384 Microplate Reader. This test was performed in duplicates. SpectraMax 190 reads 96-well and strip-well plates. Absorbance values were read at 570 nm on an automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA). The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). The reaction mixture was incubated for 5 min. 304 signal level saturation. The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. The reaction mixture was incubated for 5 min. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Description Control Panel Cuvette Chamber Back Panel Microplate Drawer The Control Panel TEMP on/off TEMP Figure 1. The membranes were. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax Gemini XS Microplate Fluorometer $2,800. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 0, 20 mmol L –1cal-cium chloride). The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the visible range. SpectraMAX 340 microtiter plate reader. 97), and each plate contained at least six positive and six negative controls. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). The concentration of Alizarin Red S staining in the samples was determined by comparing the absorbance values with those. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. Subsequently, the mixture was heated at 100 °C for 10. Microplate Readers. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). 30. The ABS reader ranges from. TGFα release was calculated as described in the Results. About GMI provides new and GMI Certified Pre-Owned scientific instrumentation and service to research universities, environmental labs, forensic investigators, biotechnology and pharmaceutical companies, hospitals, clinics and analytical laboratories, in the United. EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen. Description. Examples: “Press carefully to engage,” “Do not press down. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). The first time you replace an OEM lamp you must use the N-34-MLA. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The Spectra Catalina 340 Automated Connect 12Volt or 24Volt Catalina 340C produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. The VersaMax and SpectraMax 190 read 96-well microplates. #ABS PLUS) Methods. Male 8 pin mini Din to Female 9D. The ability of PTK787/ZK 222584 to inhibit angiogenesis was tested in an in vitro model of capillary sprout formation (64) . A. Or scan up to 384 wells at any time to determine the best assay conditions. They are for reference only. , Sunnyvale, CA, USA) at 595 nm. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. 7 macrophages were obtained from ATCC (Manassas, VA). , Sunnyvale, CA) at 490 nm. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. the SpectraMax iD3 is a fantastic plate reader for a. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). The intensity of red color formed in the assay was measured at a wavelength of 490 nm with an ELISA reader (SpectraMax 340, Molecular Device Co. View the Molecular Devices website. The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). , Fremont, CA, USA). Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). The loading equaled the mass of BSA per mass of particles. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. Absorbance was measured at 450 nm by using a SpectraMax 340 microplate spectrophotometer (Molecular Device). SPECTRAmax® 190. 2 0.